Log D

Lipophicility is another very important physical and chemical parameter of a compound, which has an impact on the solubility, absorption, membrane permeability, plasma protein binding, tissue distribution, entry into brain tissue and clearance path of the compound. The lipophicility of the compound not only affects its recognition with the target, but also affects its interaction with CYP450, HERG binding and PXR-mediated enzyme induction. The lipophicility of a compound is usually measured by Log D, and another commonly used parameter is Log P. Since most compounds will be ionized at physiological pH (7.4), the lipophicility at pH 7.4 is often measured, which is Log D7.4. logD is a log of partition of a chemical compound between the lipid and aqueous phases. The most popular lipid phase is octanol. LogP is equivalent to logD for non-ionisable compounds and represents the partition of the neutral form for ionizable compounds (and, hence, is a virtual, unmeasurable, property).

Protocol for Log D Determination

Method Shake Flask Assay
Stock Solution 10 μM
Soluent Aqueous(Buffer)/Organic(octanol)= 1:1
Positive Control ketoconazole
Analysis method LC-MS/MS
Calculation and Data delivery Log D= log (Coct/Caq)
Coct = Concentration in octanol
Caq = Concentration in aqueous buffer
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