Blood to Plasma Ratio
Usually in bioanalysis, the concentration of the tested compound refers to the concentration of the compound in plasma. For most compounds, there is no significant difference between the concentration in plasma and the concentration in whole blood. However, because the compound may selectively bind to proteins in blood cells, the plasma concentration may be different from the whole blood concentration. Therefore, the whole blood/plasma distribution ratio of the test compound helps to understand whether the compound enters and binds to the protein in blood cells. If the whole blood/plasma distribution ratio is greater than 1, it means that the compound enters the blood cells. At this time, the PK parameters calculated with the plasma concentration will not reflect the PK properties of the compound well.

Blood to Plasma Ratio Assay Protocol

Species Rat, mouse, dog, monkey, human
Test concentration 0.5 µM (other concentrations can be used)
Positive Control Methazolamide (human)
Chlorthalidone (rat and mouse)
Chloroquine (dog)
Compound required 100 µL 10mM DMSO solution or 2 mg
Replicates N=3
Analysis method LC-MS/MS
Data Delivery Mean±SEM of blood to plasma ratio
Integrate Drug Discovery Service
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